Category: Epididymis by Testicular (Page 2)

Regulation of Gamma-Glutamyl Transpeptidase: RESULTS(3)

GGT catalytic activity (Fig. 7) and GGT protein level (Fig. 8) in the initial segment decreased after 3-day EDL but returned to the sham control value after incubations with TPA but not with FCS. Presence of bFGF-Like Proteins in RTF and the Rat Epididymis Using a bFGF pAb, 16-kDa and 43-kDa protein bands were detected in rRTF (20 |jLg). However, no protein bands were detected in oRTF or no-ABP oRTF preparations using this bFGF antibody (Fig. 9A). Using a bFGF mAb, 16-, 24-, 43-, and 57-kDa proteins were detected in rRTF, and 34-kDa and 67-kDa proteins […]

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Regulation of Gamma-Glutamyl Transpeptidase: RESULTS(2)

When the initial segment after 3-day EDL was treated with 40% > 10-kDa oRTF, GGT catalytic activity returned to the sham control value (Fig. 3B). However, treatment with < 10-kDa oRTF did not recover the decreased GGT catalytic activity (Fig. 3B). GGT catalytic activity in the initial segment after 3-day EDL returned to sham control values after treatment with 20% rRTF (Fig. 3C).

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Regulation of Gamma-Glutamyl Transpeptidase: RESULTS(1)

Effects of EDL on GGT Catalytic Activity in Various Regions of the Rat Epididymis GGT catalytic activity was measured in epididymal tissue homogenate from rats that had previously undergone unilateral EDL and a sham operation. For sham-operated rats, the levels of GGT catalytic activity were initial segment > caput > corpus = cauda > testes (Fig. 1). GGT catalytic activity in the initial segment decreased by 3 days and 5 days of EDL, but did not differ significantly from that in controls after 1 day and 14 days of EDL. In the caput and corpus […]

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Regulation of Gamma-Glutamyl Transpeptidase: MATERIALS AND METHODS(4)

For immunoblotting with bFGF antibodies, the filters were blocked with 1% BSA and 1% nonfat dry milk in TBST and then incubated with 1:500 bFGF monoclonal antibody (mAb; Sigma) or with 1:400 bFGF pAb (Chemi-con International, Temecula, CA). After being washed with TBST twice, the filters were incubated with horseradish peroxidase-conjugated goat anti-mouse antibody (Santa Cruz Biotech) or horseradish peroxidase-conjugated goat anti-rabbit antibody (Santa Cruz Biotech). After extensive washing with TBST, the filters were developed by the Boehringer-Mannheim (Indianapolis, IN) chemilumines-cence method described in the manufacturer’s protocol. The Western blotting study of bFGF-like protein in […]

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Regulation of Gamma-Glutamyl Transpeptidase: MATERIALS AND METHODS(3)

RIA The concentrations of testosterone in oRTF preparations were determined using an ImmuChem testosterone 125I RIA kit (ICN Biomedicals, Costa Mesa, CA) according to the manufacturer’s protocol. asthma inhalers GGT Assay GGT catalytic activity assay was performed as described previously . Protein concentrations of samples were determined by the Bio-Rad (Richmond, CA) protein assay. For the comparative study of GGT catalytic activity among various tissues and of the effects of EDL on the GGT catalytic activity in these tissues, GGT catalytic activity was expressed as nmol/min per milligram protein. For the observation of whether RTF […]

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Regulation of Gamma-Glutamyl Transpeptidase: MATERIALS AND METHODS(2)

Preparations of Luminal Fluids from the Rat Initial Segment, Caput, and Cauda Luminal fluid from the initial segment, caput, and cauda of adult rats was collected as described previously . This luminal fluid was centrifuged at 3000 rpm in an Eppendorf microcentrifuge to remove the spermatozoa. The supernatant was collected and stored at — 80°C before use. buy antibiotics online

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Regulation of Gamma-Glutamyl Transpeptidase: MATERIALS AND METHODS(1)

Animals Normal adult male retired breeder Sprague-Dawley rats (450-550 g; Hilltop Laboratories, Philadelphia, PA) were maintained on a 12L:12D cycle, with free access to food and water, in the University of Virginia vivarium. All experiments in this study were approved by the University of Virginia Animal Welfare Committee.

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Regulation of Gamma-Glutamyl Transpeptidase Catalytic Activity and Protein Level in the Initial Segment of the Rat Epididymis by Testicular Factors: Role of Basic Fibroblast Growth Factor(2)

Previous studies have shown that macromolecules in ovine RTF (oRTF) are beneficial for maintenance of the ability to metabolize testosterone by cultured principal cells from the proximal epididymal regions , and these macromolecules can stimulate protein secretion in the initial segment of the rat epididymis . Ovine RTF contains a growth factor , and a number of growth factors are produced by the testis . Therefore, we hypothesize that testicular factors such as growth factors are involved in the regulation of GGT catalytic activity and GGT protein level in the initial segment.

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