We have now extended our work to delineate the variation of expression of DBA-binding glycans in both human and baboon endometrium, in order to determine whether there is common glycan expression among the primate species or whether interspecies variation in implantation and tro-phoblast invasion is mirrored by differences in glycosylation. In order to discriminate between different classes of terminal GalNAc, we have also used several other lectins with related binding specificities, including the agglutinins from Vicia villosa (VVA), Glycine max (SBA), Helix po-matia (HPA), and Wisteria floribunda (WFA) [12—15]. buy levaquin online
Whereas DBA binds to the fucosylated blood group A pentasaccharide (GalNAca 1,3(Fuca 1,2)-Gal(31,4GlcNAcf31,6-R) and with lower affinity to nonreducing terminal a-GalNAc , VVA binds preferentially to aGalNAc and more weakly to complex structures . HPA has a ligand repertoire that includes a broader spectrum of structures bearing terminal aGalNAc . We also included the lectin from Datura stramonium (DSA), which recognizes TV-acetyl lactosamine , in order to ascertain the likelihood that variations in terminal aGalNAc residues may arise because of alteration in one major class of core structures that bears them. In this study, we have compared the glycan composition of the luminal epithelium, which plays a crucial role in implantation, with that of the glandular compartment.