Expression of TGF-в 2 mRNA during NBUVB irradiation in human keratinocyte culture

Fig. 4A is a representative RT-PCR of extracted RNA which shows that the predicted TGF-в 2 mRNA band was detected in all samples. The combined densitometric analysis of the RT-PCR analyses is shown graphically in

Fig. 4B. TGF-в 2 mRNA levels were decreased at 800, 1,000, and 1,200 mJ/cm2 compared with the control group at 24 hr and 48 hr. However, TGF-в 2 mRNA levels were not significantly decreased at 800 mJ/cm2 at 24 hr (p>0.05).
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Fig. 4. TGF-β2 mRNA levels

Fig. 4. TGF-в 2 mRNA levels at 800, 1,000 and 1,200 mJ/cm2 compared with control group for 24 hrs and 48 hrs. (A) Representative RT-PCR showing TGF-в 2 mRNA in all samples. (B) Graphical representation of combined densitometric analyses of TGF- в 2 mRNA levels. Results are represented as the ratio of expression of TGF-в 2 to that of в-actin (n=6). *p<0.05 when compared with control group.

Expression of TGF-£3 mRNA during NBUVB irradiation in human keratinocyte culture

Fig. 5A is a representative RT-PCR of extracted RNA which shows that the predicted TGF^3 mRNA band was detected in all samples. The combined densitometric analysis of the RT-PCR analyses is shown graphically in Fig. 5B. TGF- в 3 mRNA levels were significantly increased at 800, 1,000, and 1,200 mJ/cm2 at 24 hr (p<0.05). How¬ever, TGF- в 3 mRNA levels did not differ from expression levels compared to control at 800, 1000, and 1,200 mJ/cm2 at 48 hr (p>0.05).
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Fig. 5. TGF-β3 mRNA levels

Fig. 5. TGF- в 3 mRNA levels at 800, 1,000 and 1,200 mJ/cm2 compared with control group for 24 hr and 48 hr. (A) Representative RT-PCR showing TGF- в 3 mRNA in all samples. (B) Graphical representation of combined densitometric analyses of TGF- в 3 mRNA levels. Results are represented as the ratio of expression of TGF-в3 to that of в-actin. (n=6). *p<0.05 when compared with control group.