Transforming growth factor в (TGF- в)

Expression of TGF- в 1 protein during NBUVB in human keratinocyte culture

As shown in Fig. 1, a representative ELISA of human keratinocyte, TGF- в 1 protein levels were significantly increased at 800, 1000, and 1200 mJ/ cm2 compared with the control group at 48 hr. However, TGF- в 1 protein levels were decreased at 1,000 and 1,200 mJ/cm2 at 24 hr compared to control (p < 0.05). TGF- в 1 protein levels were not significantly increased or decreased at 800 mJ/cm2 at 24 hr compared to control (p>0.05).

Fig. 1. TGF-β1 protein levels

Fig. 1. TGF- в 1 protein levels at 800, 1,000 and 1,200 mJ/cm2 for 24 hr and 48 hr when compared with the control group. TGF- в 1 protein levels in supernatant was determined by ELISA assay (duplicates). Values are the mean±SD of 6 samples in 2 expriments. *p<0.05 when compared with the control group.

Expression of TGF- /3 2 protein during NBUVB in human keratinocyte culture

As shown in Fig. 2, TGF-в 2 protein levels were significantly decreased at 1,000 and 1,200 mJ/cm2 compared with the control group at both 24 hr and 48 hr. However, TGF- в 2 protein levels were not- significantly decreased at 800 mJ/cm2 at 24 hr and 48 hr compared to control (p>0.05).
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Fig. 2. TGF-β2 protein levels

Fig. 2. TGF-в 2 protein levels at 800, 1,000 and 1,200 mJ/cm2 for 24 hrs and 48 hrs when compared with the control group. TGF- в 2 protein levels in human kera- tinocyte supernatant was determined by ELISA assay. Values are the mean±SD of 6 samples in 2 expriments. *p<0.05 when compared with the control group.

Expression of TGF-в mRNA during NBUVB irradiation in human keratinocyte culture

Fig. 3A is a representative RT-PCR of extracted RNA which shows that the predicted TGF- в 1 mRNA band was detected in all samples. The com-bined densitometric analysis of the RT-PCR analyses is shown graphically in Fig. 3B. After NBUVB irra-diation, TGF- в 1 mRNA levels were increased at 800, 1,000, and 1,200 mJ/cm2 compared with the control group (p<0.05) at 24 hr and 48 hr. However, TGF- в 1 mRNA levels were not signifi¬cantly increased at 800 mJ/cm2 at 24 hr (p>0.05). Both TGF- в 1 mRNA and protein levels were significantly increased at 48 hr.
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Fig. 3. TGF-β1 mRNA levels

Fig. 3. TGF- в 1 mRNA levels at 800, 1,000 and 1,200 mJ/cm2 compared with control group for 24 hr and 48 hr. (A) Representative RT-PCR showing TGF^1 mRNA in all samples. (B) Graphical representation of combined densitometric analyses of TGF- в 1 mRNA levels. Results are represented as the ratio of expression of TGF-в 1 to that of в-actin (n=6). *p<0.05 when compared with control group.