Sample Collection

Nonpregnant human endometrial specimens were collected from premenopausal women undergoing hysterectomy for indications other than those of malignancy (n = 12). The stage of the cycle of each tissue was estimated by the time of the last menstrual period and was confirmed by histological dating performed by experienced clinical pathologists. Tissue was also obtained from postmenopausal hysterectomy patients (n = 13). Endometrial biopsy tissue from 26 oocyte donors at Days 4 and 10 post-LH surge of a monitored (nontreatment) cycle was supplied by Dr. G. Ndukwe at Queen’s Medical Centre, Nottingham, UK. Buy Advair Diskus Online

Human first-trimester decidua was obtained by dissection from the products of conception following therapeutic terminations of pregnancy at the Queen’s Medical Centre (n = 12, gestation 7-12 wk). The duration of pregnancy was calculated from the date of the last menstrual period. A rare second-trimester hysterotomy specimen (from a therapeutic termination of pregnancy) was also studied (n = 1). Paraffin-embedded sections of baboon uterus from pregnant, pseudopregnant, and cycling animals were obtained as previously described. Additional tissues from pregnant baboons were kindly provided by Dr. Bambra (Institute for Primate Research, Nairobi, Kenya). All human tissue was obtained with the approval of the Ethics Committee of the Queen’s Medical Centre, Nottingham. Animal investigations were conducted in accordance with the Guiding Principles for the Care and Use of Research Animals promulgated by the Society for the Study of Reproduction, and the studies were approved by the Animal Care committees at the University of Illinois at Chicago and the Institute for Primate Research, Nairobi.