For immunoblotting with bFGF antibodies, the filters were blocked with 1% BSA and 1% nonfat dry milk in TBST and then incubated with 1:500 bFGF monoclonal antibody (mAb; Sigma) or with 1:400 bFGF pAb (Chemi-con International, Temecula, CA). After being washed with TBST twice, the filters were incubated with horseradish peroxidase-conjugated goat anti-mouse antibody (Santa Cruz Biotech) or horseradish peroxidase-conjugated goat anti-rabbit antibody (Santa Cruz Biotech). After extensive washing with TBST, the filters were developed by the Boehringer-Mannheim (Indianapolis, IN) chemilumines-cence method described in the manufacturer’s protocol. The Western blotting study of bFGF-like protein in the luminal fluid from various epididymal regions was repeated twice, and the other experiments were repeated on samples from at least three individual experiments. buy flovent inhaler

Statistical Analysis

Analysis of variance was used to test the statistical significance of the effects of EDL or treatments on the GGT catalytic activity among different groups. Results were presented as means ± SEM from at least three individual experiments in each group. Data were examined by one-way analysis of variance, followed by Tukey’s test. Results were considered significantly different at p < 0.05.