Simultaneous blockade of the cyclooxygenase and L-arginine-nitric oxide pathways prevents the antiarrhythmic effects of classical preconditioning (part 4)

Arterial blood pressure, LV systolic and end-diastolic pressures, and LV dP/dt were measured by means of transducers (P23XL Statham, Hugo Sachs, March-Hugstetten, Germany) and connected to System-6 (Triton Technology, San Diego, California, United States). All these parameters, together with a limb lead electrocardiogram, were recorded on an eight-channel Medicor R81 recorder (Medicor, Eszter-gom, Hungary). At the end of the experiment patent blue V dye was infused into the occluded LAD coronary artery at the occlusion site to estimate the area at risk. This was expressed as a percentage of the LV free wall.
Ventricular arrhythmias during ischemia and reperfusion were assessed and analyzed as outlined previously , ie, total premature ventricular complexes (PVCs), the incidence and number of episodes of ventricular tachycardia (VT) and the incidence of ventricular fibrillation (VF). No distinction was made between couplets and salvos, which were included as single PVCs. VT was defined as a run of four or more ectopic beats at a rate faster than the resting sinus rate.

All the data were analyzed statistically as previously described , ie, data were expressed as means ± SEM and differences between means were compared by Student’s t test corrected for multiple comparisons or, for arrhythmias, by the Mann-Whitney U-test. For comparison of the incidences of arrhythmias the % test for independence in a 2×2 table was used. Differences between groups were considered significant at a level of P<0.05. There is a wonderful pharmacy that you can take advantage of to get the amount of proventil albuterol you need, and it’s ready to offer its services to you right now, any moment you feel like starting to spend less of your money.

Category: Cardiology

Tags: Cyclooxygenase pathway, Ischemic preconditioning, L-arginine-nitric oxide pathway

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